16S rRNA Gene sequencing: A practical approach to confirming the identity of food borne bacteria
نویسندگان
چکیده
منابع مشابه
DETECTION OF BACTERIA BY AMPLIFYING THE 16S rRNA GENE WITH UNIVERSAL PRIMERS AND RFLP
Background: There is a conserved portion in the 16S rRNA gene of bacteria which can be amplified by the universal PCR method. This fragment is 996 bp in length. In this method, only one set of universal primers is used for the amplification of the conserved region of the 16S rRNA gene, in common bacterial pathogens. Therefore, using the universal PCR method, these bacteria are detectable on...
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15 صفحه اول16S rRNA gene sequencing on a benchtop sequencer: accuracy for identification of clinically important bacteria
AIMS Test the choice of 16S rRNA gene amplicon and data analysis method on the accuracy of identification of clinically important bacteria utilizing a benchtop sequencer. METHODS AND RESULTS Nine 16S rRNA amplicons were tested on an Ion Torrent PGM to identify 41 strains of clinical importance. The V1-V2 region identified 40 of 41 isolates to the species level. Three data analysis methods wer...
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ژورنال
عنوان ژورنال: Ife Journal of Science
سال: 2019
ISSN: 0794-4896
DOI: 10.4314/ijs.v21i3.2